《植物生理学报》 2015, 51(7): 1151-1156

不同供体材料对非洲菊胚珠离体培养的影响

单芹丽¹, 王继华¹, 吴丽芳², 王丽花¹, 汪国鲜², 李绅崇^1,*, 杨春梅^2,*

¹云南省农业科学院花卉研究所, 国家观赏园艺工程技术研究中心, 昆明650205; ²玉溪云星生物科技有限公司, 云南玉溪652604

通信作者：李绅崇；E-mail: 693243551@qq.com, 1577225885@qq.com；Tel: 13759115792, 13368713967

摘　要：

以非洲菊胚珠为试材, 研究供体材料的基因型、取材季节、发育时期和小花类型对胚珠培养的影响。结果表明: 45个非洲菊基因型胚珠诱导培养90 d时, 有29个基因型能诱导出不定芽, 不定芽的诱导率超过10%的基因型仅有3个, 不定芽诱导率最高达17.7%。胚珠诱导不定芽的效果为: 夏季>春季>秋季>冬季; 非洲菊花药未吐粉前, 花序越成熟, 不定芽诱导率越高, 诱导时间越短; 舌状花的诱导效果略好于管状花。四因素对非洲菊不定芽诱导效果的主次关系是基因型>季节>发育时期>小花类型, 基因型是决定非洲菊不定芽诱导效果的重要因素, 小花类型对不定芽的诱导效果没有显著影响。

关键词：胚珠培养; 基因型; 季节; 发育时期; 小花类型

收稿：2015-06-01   修定：2015-06-30

资助：国家观赏园艺工程技术研究中心项目(2012FU125X10)、云南省战略性新兴产业发展专项资金项目(昆财企一[2013]118号)和云南省科技厅科技创新强省计划项目(2014AB014)。

Effects of Different Donor Material on Ovule Culture in Vitro in Gerbera jamesonii

SHAN Qin-Li¹, WANG Ji-Hua¹, WU Li-Fang², WANG Li-Hua¹, WANG Guo-Xian², LI Shen-Chong^1,*, YANG Chun-Mei^2,*

¹Flower Research Institute, Yunnan Academy of Agricultural Sciences, National Engineering Research Center for Ornamental Horticulture, Kunming 650205, China; ²Yuxi Yunxing Biotechnology Co., Ltd., Yuxi, Yunnan 652604, China

Corresponding author: LI Shen-Chong; E-mail: 693243551@qq.com, 1577225885@qq.com; Tel: 13759115792, 13368713967

Abstract:

Ovules of Gerbera jamesonii were selected as materials. The effects of genotype, season, developmental stage and flower type on the induction of adventitious buds were studied. Results showed that ovules of 45 genotypes were cultured at 90 days, there were 29 genotypes could induce adventitious buds, and adventitious bud induction rate of only 3 genotypes were more than 10%. The induction rate of adventitious buds highest reached 17.7%. The relationship of effects that ovule induced adventitious buds was summer>spring>autumn>winter; before anther spit out pollen, inflorescence more mature, the higher the rate of adventitious buds, the shorter induction time; the induction effect of ray florets were slightly better than tubular flowers. The relationship of primary and secondary effects that 4 factors on adventitious buds induction was genotype>season>developmental stages>flower type, Gerbera genotype was an important factor in determining the induction effect of adventitious buds, flower type had no significant effect on the induction of adventitious buds.

Key words: ovule culture; genotype; season; growth period; flower type